ABSTRACT
Objective To compare the prevalence of overweight and obesity in urban children in Chongqing city with 3 different refe-rences and find the appropriate reference for obesity-related study in children.Methods A cross-sectional study including 12 534 urban children aged 2-18 years old was performed.The data were selected from 3 districts in Chongqing city by multistage random cluster sampling.Children′s body height and weight were measured with unique method by trained doctors.Body mass index (BMI,kg/m2) was calculated for each child.Overweight and obese children were defined according to WHO weight-for-height reference values of urban children (1995,standard 1), International Obesity Task Force (IOTF) age-and gender-specific BMI cut-offs (2000,standard 2),and National Center for Health Statistics / Center for Disease Control and Prevention(NCHS/CDC)age-and gender-specific BMI cut-offs(2000,standard 3),respectively.Results The prevalence of overweight in all children was 13.0%,9.8%,and 9.8%,and the prevalence of obesity in all children was 9.8%,2.9%,and 6.0% by using the standard 1,standard 2 and standard 3,respectively,which showed significant differences between the references (Pa
ABSTRACT
In order to establish the optimum condition for purifica tion of the nucleoprotein(NP) of rabies virus by immunoaffinity chromatography, the efficient and non-denaturative eluents(Mg-el) was obtained by using ELISA elution model; furthermore, it didn't damage the activity of NP. Two kind of NPs , expressed by recombinant vaccinia virus (rVac-N) and recombinant baculovirus (BRN), were purified by a Sepharose CL 4B column and a 2C12- Sepharose 4B colum n. By Western-blot and SDS-PAGE, high purity and good antigenical intact NPs w ere identified. The purified ribonucleoprotein (RNP) of rabies virus 5aG strain was also obtained. After immunized with NP and RNP, mice developed a strong anti -nucleoprotein response and were protected against a lethal challenge of rabies virus CVS strain. There were not difference been observed among the mice immuni zed with different purified protein. These data indicate that the NPs are antige nical and immunogenical comparable to the authentic rabies RNP and therefore pre sent a potential source of an effective ,safe and economical subunit vaccine.